Chemistry of Life: pH and Buffers

Chemistry of Life: pH and Buffers

Explain why the regulation of pH is necessary for biological systems.
Use a chemical indicator to determine the pH of a solution.
Analyze prepared buffer solutions.
Perform calculations needed to prepare a buffer from a weak acid and its conjugate base.

Materials

Materials

Sodium Phosphate Buffer, pH 4, 25 mL
Sodium Phosphate Buffer, pH 6, 50 mL
Sodium Phosphate Buffer, pH 8, 25 mL
Sodium Hydroxide (NaOH), 1 M, 30 mL
Hydrochloric Acid (HCl), 1 M, 15 mL
Bogen’s Universal Indicator, 7 mL
Bogen’s Universal Indicator Color Chart
7 Graduated Dropping Pipets, 3 mL
Jelly Tray, 4 × 5 Wells

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