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Thin Layer Chromatography of Spinach Extract

Thin Layer Chromatography of Spinach Extract

  • Perform a separation of plant pigments using thin-layer chromatography.
  • Determine the relative polarity of plant pigments based on the Rf value of the TLC plate.
  • Calculate Rf values of plant pigments.
  • Explain how thin-layer chromatography works.

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Materials

  • Thin-Layer Chromatography Plates
  • Octanol
  • Acetone
  • Capillary Tubes
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